g-Trap Ni IDA瓊脂糖快速流動色譜柱可直接用于基于固定金屬離子親和色譜（IMAC）的蛋白質(zhì)純化。

IMAC基于過渡金屬陽離子如Ni2+、Co2+與蛋白質(zhì)殘基組氨酸、半胱氨酸以及某種程度上色氨酸的相互作用。在G-Trap Ni IDA瓊脂糖快速流中，Ni2+使用與樹脂共價(jià)結(jié)合的亞氨基二乙酸基團(tuán)（IDA）固定在交聯(lián)的6%瓊脂糖上。 

g-Trap Ni IDA瓊脂糖快速流專為純化融合了6x組氨酸（6XHis）標(biāo)簽的重組蛋白而設(shè)計(jì)。

其他G-Trap IDA瓊脂糖快速流動色譜柱提供1 ml和5 ml柱體積g-Trap Co IDA瓊脂糖快速流動。人們可以根據(jù)需要在合適柱之間進(jìn)行選擇。

G-Trap Ni IDA瓊脂糖快速流動樹脂和柱的特性如下所示。

g-Trap Ni IDA瓊脂糖快速流動樹脂

Matrix: Cross-linked agarose beads, 6%

Mean particle size: 90 μm

Chelating Agent: Iminodiacetic acid

Active group: Ni2+

Metal ion density: 20-40 μmol /ml

Binding Capacity: 5-10mg His-tagged protein/ml medium

pH stability Working Range: 3-12

pH stability Cleaning-in-Place (CIP): 2-14

Maximum Flow Velocity: 2-14

Recommended Flow rate: 1 ml/min and 5 ml/min for 1 ml and 5 ml column respectively

Exclusion limit(globular proteins): 4 x 106

Chemical Stability: Stable to all commonly used aqueous buffers: 8 M urea, 6 M guanidine hydrochloride

Storage Conditions: 2 to 8°C, 20% Ethanol

G-Trap™ Ni IDA Agarose Fast Flow, 1 ml columns

Column Volume: 1 ml

Columns Dimension: 0.7 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

G-Trap™ Ni IDA Agarose Fast Flow, 5 ml columns

Column Volume: 5 ml

Columns Dimension: 1.6 x 2.5 cm

Column Harware Pressure Limit: 0.5 MPa

Column Hardware: Polypropylene (Biocompatible)

應(yīng)用程序:

• g-Trap Ni IDA瓊脂糖快速流動色譜柱可直接用于基于固定金屬離子親和色譜（IMAC）的蛋白質(zhì)純化。g-Trap Ni IDA瓊脂糖快速流專為純化融合了6x組氨酸（6XHis）標(biāo)簽的重組蛋白而設(shè)計(jì)。